Fluorescent staining of microfilaments with heavy meromyosin labeled with N-(7-dimethylamino-4-methylcoumarinyl) maleimide.
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چکیده
منابع مشابه
Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells
We purified actin antibodies by affinity chromatography from the serum of rabbits immunized with glutaraldehyde-fixed chicken gizzard actin filaments and used this anti-actin to localize actin in myofibrils and fixed cultured cells at each stage of the cell cycle. By double immunodiffusion the anti-actin reacted with both smooth and skeletal muscle actin. Several blocking and absorption experim...
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Difference spectra induced by ATP and its analogues of the ultraviolet absorption of heavy meromyosin around 280 rnp were measured by the double cell method. The difference spectrum induced by ATP showed two peaks, at 281 and 289 rnp, and a shoulder near 300 mp. The maximum value of the difference molar extinction coefficient, AE, at 289 mp, was 5000 f 300 Me1 cm-l. The difference spectrum caus...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.4.6989895